Abstract:
Background: The objective of the study was to characterize homoeopathic nanomedicine Aurum metallicum and evaluate its biocompatibility,
to explore its possible application as injectables. Metal‑based homoeopathic medicine, Aurum metallicum, was chosen as a model drug
and the haemocompatibility of the drug at three different potencies 6C, 30C, and 200C were studied to find the justification of the drug as
an injectable candidate for clinical application. Methods: The model drug Aurum metallicum at the three potencies was characterized by
dynamic light scattering (DLS), zeta potential, field emission scanning electron microscopy (FESEM), and energy dispersive X‑ray analysis.
Hemocompatibility of the homoeopathic medicine was performed by haemolysis assay. Red blood cell obtained from fresh human blood
by centrifugation was incubated with Aurum metallicum. Haemoglobin release was measured using UV‑vis spectrophotometer at 540 nm.
Results: The DLS and FESEM studies show a decrease of particle size with increasing potency. The zeta potential values show a fairly constant
value measured at an interval of 10 days. The haemolysis percentage for 6C, 30C, and 200C was 9.73%, 8.16%, and 0.73%, respectively.
Conclusion: The nanomedicine Aurum metallicum was nontoxic at all doses of 6C, 30C, and 200C. The haemolytic percentage also shows
that 200C is nonhemolytic, showing haemolysis <2% as per the American Society for Testing and Materials guidelines. The undertaking of
larger controlled and in‑depth qualitative studies is warranted.
