Abstract:
In this study,we have chosen HPTLC (High Performance Thin Layer Chromatography) as a method
of analysis to develop a standard procedure based on fingerprinting characteristics for the
evaluation of homreopathic formulations. A simple and accurate HPTLC method has been developed
for the quantification of brucine,one of the chief active chemical components of Nux vomica (along
with strychnine),and the fingerprinting of the in-house mother tincture of Nux vomica, considered
here to be a standard, with that of different marketed samples available from manufacturers of
homreopathic medicines in India. This HPTLC method was quantitatively evaluated in terms of
stability, repeatability, and accuracy.