Application of HPTLC in the standardization of a homoeopathic mother tincture of nux vomica

Abstract

In this study we have chosen HPTLC( High Performance thin layer chromatography) as a method of analysis to develop a standard procedure based on fingerprinting characteristics for the evaluation of homoeopathic formulations. A simple and accurate HPTLC method has been developed for the quantification of brucine, one of the chief active chemical component of Nux vomica (along with strychnine) and the fingerprinting of the in-house mother tincture of Nux vomica considered here to be a standard with that of different marketed samples available from manufacturers of homoeopathic medicines in India. This HPTC method was quantitatively evaluated in terms of stability reputability and accuracy.