Application of HPTLC in the Standardization of a Homoeopathic Mother Tincture of Nux Vomica

Abstract

In this study,we have chosen HPTLC (High Performance Thin Layer Chromatography) as a method of analysis to develop a standard procedure based on fingerprinting characteristics for the evaluation of homreopathic formulations. A simple and accurate HPTLC method has been developed for the quantification of brucine,one of the chief active chemical components of Nux vomica (along with strychnine),and the fingerprinting of the in-house mother tincture of Nux vomica, considered here to be a standard, with that of different marketed samples available from manufacturers of homreopathic medicines in India. This HPTLC method was quantitatively evaluated in terms of stability, repeatability, and accuracy.